花青素合成调控转录基因作为直观标记的载体构建及其在玉米幼胚中的瞬时表达

Author(s)
Zhao, Xingmei
Wu, Shubiao
Wang, Yixue
Cui, Guimei
Wang, Xiaoqing
Du, Jianzhong
Wang, Xiaoli
Shang, Yongjin
Sun, Yi
Publication Date
2014
Abstract
采用花青素调控因子C1/Bperu分别与玉米胚特异性启动子Glb1和组成型启动子CaMV35S构建成植物转化载体pGlb1CB和p35SCB，并利用基因枪转化方法，将重组表达载体转入玉米幼胚。显微观察结果证实，这两个载体均能在玉米幼胚细胞中瞬时表达。用花青素作为标记基因不仅可以在一定程度上减少公众对转基因生物安全性方面的担忧，而且可以帮助直观地从转化当代和后代种子中通过颜色标记筛选到转化籽粒，从而可以大大简化筛选程序，提高效率，节约检测成本。
Citation
Shengwu Jishu Tongbao, 2014(4), p. 77-82
ISSN
1002-5464
Link
https://hdl.handle.net/1959.11/19091
Language
zh
Publisher
Zhongguo Nongye Kexueyuan
Title
花青素合成调控转录基因作为直观标记的载体构建及其在玉米幼胚中的瞬时表达
Type of document
Journal Article
Entity Type
Publication
Abstract
Anthocyanin regulatory factor C1/Bperu genes are constructed respectively with a maize embryo specific promoter GLB1 and a constitutive promoter CaMV35S into plant transformation vectors pGlb1CB and p35SCB, and the recombinant expression vectors were introduced into maize embryos by particle bombardment. Microscopic observation confirmed that the two vectors were actively expressed in maize immature embryo cells. The use of anthocyanins as a marker gene not only could reduce the safety concerns from the public over GMO substantially, but could also be used as a visual indicator for the selection of transgenic plants, which could greatly simplify the screening procedures, improve the efficiency and reduce the costs.

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