Evaluation of κ-casein and growth hormone gene polymorphism in native Dalagh sheep

Title
Evaluation of κ-casein and growth hormone gene polymorphism in native Dalagh sheep
Publication Date
2011-12-31
Author(s)
Azari Ahani, M
Yousefi, S
Dehnavi, E
( partner investigator )
OrcID: https://orcid.org/0000-0001-8238-6290
Email: edehnavi@une.edu.au
UNE Id une-id:edehnavi
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Narodne Polnohospodarske a Potravinarske Centrum Vyskumny Ustav Zivocisnej Vyroby Nitra, Research Institute of Animal Production Nitra
Place of publication
Slovakia
UNE publication id
une:1959.11/75035
Abstract

In this research for studying genetic polymorphism of κ-casein (CSN3) and growth hormone (GH) genes as candidate genes for marker-assisted selection in different domestic livestock species, blood samples (120 samples) were collected from Dalagh sheep randomly. DNA was extracted from blood samples and a 416 bp fragment from exon 4 of the CSN3 gene and a 365 bp region from exon 5 of the GH gene were amplificated by polymerase chain reaction. PCR products were analyzed using single strand conformation polymorphism (SSCP) method on a polyacrilamide gel. Results indicate that there are two (K1=0.92 and K2=0.08) and three (G1=0.84, G2=0.13 and G3=0.03) conformational patterns for CSN3 and GH genes, respectively.

Link
Citation
Slovak Journal of Animal Science, 44(4), p. 129-133
ISSN
1338-0095
1337-9984
Start page
129
End page
133

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