Molecular changes to the rat renal cotransporters PEPT1 and PEPT2 due to ageing

Title
Molecular changes to the rat renal cotransporters PEPT1 and PEPT2 due to ageing
Publication Date
2019-02
Author(s)
Alghamdi, Othman A
King, Nicola
Andronicos, Nicholas M
( author )
OrcID: https://orcid.org/0000-0001-5881-2296
Email: nandroni@une.edu.au
UNE Id une-id:nandroni
Jones, Graham L
( author )
OrcID: https://orcid.org/0000-0002-6435-1542
Email: gjones2@une.edu.au
UNE Id une-id:gjones2
Chami, Belal
Witting, Paul K
Moens, Pierre D J
( author )
OrcID: https://orcid.org/0000-0003-3121-5306
Email: pmoens@une.edu.au
UNE Id une-id:pmoens
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Springer New York LLC
Place of publication
United States of America
DOI
10.1007/s11010-018-3413-x
UNE publication id
une:1959.11/26648
Abstract
Renal PEPT1 and PEPT2 cotransporters play an important role in the balance of circulating body oligopeptides and selected peptidomimetic drugs. We aim to comprehensively characterise age-related changes of the renal PEPT cotransporters at the gene, protein, and functional level. Brush border membrane vesicles (BBMV) and outer medulla membrane vesicles (OMMV) were isolated from the kidneys of young, middle-aged and old rats. The protein expression of PEPT1 was not only increased in BBMV from old rats, but PEPT1 also appeared in OMMV from middle-aged and old rats. SLC15A1 gene expression in the renal cortex increased in middle-aged group. PEPT2 protein expression was not only increased with ageing, but PEPT2 also was found in BBMV from middle-aged and old groups. SLC15A2 gene expression in the renal outer medulla increased in the old group. These changes in the expressions and localisations of PEPT1 and PEPT2 could explain the changes to transport activity in BBMV and OMMV. These findings provide novel insights that would be useful for maintaining protein nutrition and optimising the delivery of some peptidomimetic drugs in elderly individuals.
Link
Citation
Molecular and Cellular Biochemistry, 452(1-2), p. 71-82
ISSN
1573-4919
0300-8177
Pubmed ID
30019300
Start page
71
End page
82

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