A Loop Isothermal Amplification Assay to Detect Fowl Adenovirus -8 in a Variety of Poultry Materials

Title
A Loop Isothermal Amplification Assay to Detect Fowl Adenovirus -8 in a Variety of Poultry Materials
Publication Date
2015
Author(s)
Renz, Katrin
Achari, Robin
Walkden-Brown, Steve W
( author )
OrcID: https://orcid.org/0000-0002-0638-5533
Email: swalkden@une.edu.au
UNE Id une-id:swalkden
Katz, Margaret E
Editor
Editor(s): Peter Selle
Type of document
Conference Publication
Language
en
Entity Type
Publication
Publisher
University of Sydney
Place of publication
Sydney, Australia
UNE publication id
une:19715
Abstract
A variety of molecular tests to detect avian adenoviruses are available, but they are either time-consuming or not specific to Fowl adenovirus serotype 8 (FAdV-8). Therefore, the aim was to develop a loop isothermal amplification (LAMP) assay to specifically detect FAdV-8 in a variety of poultry samples, particularly dust, faeces and litter. The LAMP assay is a particularly rapid, robust and a low cost molecular test yet with a sensitivity comparable with the real-time PCR technique. Samples were analysed from chickens experimentally infected with FAdV-8 and derived from both tissues and environmental samples, including dust, litter and faeces. The LAMP results matched with those obtained from a generic FAdV real-time PCR test and the LAMP test has shown no cross-reactivity with FAdV serotypes 2, 9, or 11.
Link
Citation
Proceedings of the Australian Poultry Science Symposium, v.26, p. 171-174
ISSN
1034-6260
1034-3466
Start page
171
End page
174

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