Agricultural production (especially intensive rice production) is a primary income source for over 2.0×107 people in the Vietnamese Mekong River Delta. However, adverse climate change impacts, socio-economic change, and high dependence on farm inputs for intensive production constrain the longer-term sustainability of rice systems. Government and agribusiness actors are encouraging more farmers to grow non-rice crops and supporting the upscaling of alternative crops to paddy rice. We used a qualitative approach to investigate the value chain characteristics, as well as constraints and opportunities of alternative crops via two case studies (baby corn and honeydew melon) in An Giang and Hau Giang provinces, Vietnam. Data collection involved focus group discussions with local farmers and interviews with farmers and industry experts. Thematic analysis was used to compile the findings, and the results were validated with local government staff. The baby corn value chain featured on-going and stable market demand (including value-addition) and better vertical coordination (e.g., written contracts and financial support). The honeydew melon value chain featured positive relationships between farmers and traders despite lessdeveloped vertical coordination. There are opportunities for value chain engagement through product quality certification, value-addition, and accessing high-value domestic and export markets. However, farmers require crop-specific and generic support from private and public sectors. Increased labour requirements and limited access to finance and credit limit value chain participation. Upscaling and marketing alternative crops can enhance farmer profitability and support non-farming agricultural business establishment, economic growth, and community development. Efficient value chains will be critical to ensure the adoption of alternative crops and development of crop-specific agribusiness models. These findings can inform policy-makers and change facilitators in designing targeted interventions to support the adoption of alternative crops in the study area as well as in Vietnam and globally