Age-related neurodegenerative disease associated pathways identified in retinal and vitreous proteome from human glaucoma eyes

2017-10-04

Mirzaei, Mehdi

Gupta, Veer B

Chick, Joel M

Greco, Todd M

Wu, Yunqi

Chitranshi, Nitin

Vander Wall, Roshana

Hone, Eugene

Deng, Liting

Dheer, Yogita

Abbasi, Mojdeh

Rezaeian, Mahdie

Braidy, Nady

You, Yuyi

Hosseini Salekdeh, Ghasem

Haynes, Paul A

Molloy, Mark P

Martins, Ralph

Cristea, Ileana M

Gygi, Steven P

Graham, Stuart L

Gupta, Vivek K

Journal Article

en

Publication

Nature Publishing Group

United Kingdom

10.1038/s41598-017-12858-7

une:1959.11/71678

Glaucoma is a chronic disease that shares many similarities with other neurodegenerative disorders of the central nervous system. This study was designed to evaluate the association between glaucoma and other neurodegenerative disorders by investigating glaucoma-associated protein changes in the retina and vitreous humour. The multiplexed Tandem Mass Tag based proteomics (TMT-MS3) was carried out on retinal tissue and vitreous humour fluid collected from glaucoma patients and age-matched controls followed by functional pathway and protein network interaction analysis. About 5000 proteins were quantified from retinal tissue and vitreous fluid of glaucoma and control eyes. Of the differentially regulated proteins, 122 were found linked with pathophysiology of Alzheimer’s disease (AD). Pathway analyses of differentially regulated proteins indicate defects in mitochondrial oxidative phosphorylation machinery. The classical complement pathway associated proteins were activated in the glaucoma samples suggesting an innate inflammatory response. The majority of common differentially regulated proteins in both tissues were members of functional protein networks associated brain changes in AD and other chronic degenerative conditions. Identification of previously reported and novel pathways in glaucoma that overlap with other CNS neurodegenerative disorders promises to provide renewed understanding of the aetiology and pathogenesis of age related neurodegenerative diseases.

link

Scientific Reports, v.7, p. 1-16

2045-2322

1

16

Attribution 4.0 International