Vouraki, S

Gelasakis, A I

Alexandri, P

Boukouvala, E

Ekateriniadou, L

Banos, G

Arsenos, G

2018-08-28

<p>The objectives were to determine the genetic profile of scrapie codons 146, 211 and 222 in three dairy goat breeds in Greece and assess its impact on milk production and udder health. A total of 766 dairy goats from seven farms were used. Animals belonged to two native (Eghoria; n=264 and Skopelos; n=287) and one foreign breed Damascus; n=215. Genomic DNA was extracted from individual blood samples. Polymorphisms were detected using RealTime PCR analysis and four different Taqman assay mixes. Milk production and udder health traits of individual animals were recorded monthly for two consecutive milking periods. The former included daily and lactation milk yield and composition; fat, protein, lactose and solids-not-fat (SNF) content. Udder health traits included udder fibrosis, abscess and asymmetry, milk somatic cell count, and number of colony-forming units. Genotypic, allelic and haplotypic frequencies were calculated and genetic distances between breeds were estimated using fixation index FST with Arlequin v3.1 software. The effects of genotype and allele on the studied traits were assessed with mixed linear models with R package ‘lme4’. All resistance-associated alleles (146S, 146D, 211Q and 222K) were detected in the studied goats. Significant differences (P<0.01) in genetic profile were observed between the two native breeds and Damascus. Alleles 222K and 146S had the highest frequency (ca.6%) in the native and Damascus breeds, respectively. Resistance-associated alleles and genotypes did not affect animal traits (P>0.01) except for a mild adverse effect of codon 146 on milk SNF in native goats. Results suggest that separate breeding programs should apply to native and Damascus goats aiming at enhancing scrapie resistance. Selection for the latter is not expected to compromise animal productivity and udder health. This work was funded by the SOLID project (FP7-266367) and Onassis Foundation.</p>

Book of Abstracts of the 69th Annual Meeting of the European Federation of Animal Science, v.24, p. 303-303

9789086863235

9789086868711

1382-6077

https://hdl.handle.net/1959.11/64883

en

Wageningen Academic Publishers

Profile of PRNP gene in 3 dairy goat breeds and association with milk production and udder health

Conference Publication

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