Temperament has important impacts on on-farm profitability through its impact on other production traits like reproduction, production and meat quality in addition to its direct impact on the welfare of farm staff. The Southern Multibreed project to date has recorded over 9,800 flight time (FT) and crush score (CS) phenotypes to create a multibreed reference population for cattle temperament. This paper also examines the impact animal handling due to extensive trait recording has on the quality of temperament measures using genetic parameters. The heritabilities for FT were moderate in steers (0.27-0.31) and low to moderate in heifers (0.15-0.25). The heritabilities for CS were moderate for both steers (0.24-0.25) and heifers (0.29-0.36). The phenotypic variance for FT decreased between the weaning and yearling stages in steers but increased in heifers. The phenotypic variance for CS increased between the weaning and yearling stages in steers but decreased in heifers. The genetic correlation between FT measures at weaning and yearling stages was 0.95 in steers and 0.69 in heifers. The correlations between weaning and yearling stages for CS were 0.72 and 0.59 in steers and heifers, respectively. The correlations between steer and heifer FT and CS at the weaning stage were 0.95 and 0.87, respectively. At the yearling stage these correlations were 0.69 and 0.84. These results suggest that the heifers assimilated to the crush environment as time progressed due to extensive phenotypic recording while the steers displayed their inherent behaviour when they were exposed to the stress associated with the new environment when entering the feedlot. These outcomes indicate it is important that animals are in an environment where learnt behaviours don’t impact the expression of inherent behaviour when temperament traits are recorded.