Bacillus amyloliquefaciens CECT 5940 improves performance and gut function in broilers fed different levels of protein and/or under necrotic enteritis challenge

Title
Bacillus amyloliquefaciens CECT 5940 improves performance and gut function in broilers fed different levels of protein and/or under necrotic enteritis challenge
Publication Date
2021-03
Author(s)
Gharib-Naseri, Kosar
( author )
OrcID: https://orcid.org/0000-0003-1740-7889
Email: kgharib2@une.edu.au
UNE Id une-id:kgharib2
Dorigam, Juliano C P
Doranalli, Kiran
Morgan, Natalie
( author )
OrcID: https://orcid.org/0000-0002-9663-2365
Email: nmorga20@une.edu.au
UNE Id une-id:nmorga20
Swick, Robert A
( author )
OrcID: https://orcid.org/0000-0003-3376-1677
Email: rswick@une.edu.au
UNE Id une-id:rswick
Choct, Mingan
Wu, Shu-Biao
( author )
OrcID: https://orcid.org/0000-0002-1790-6015
Email: swu3@une.edu.au
UNE Id une-id:swu3
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Zhongguo Xumu Shouyi Xuehui, Chinese Association of Animal Science and Veterinary Medicine
Place of publication
China
DOI
10.1016/j.aninu.2020.05.004
UNE publication id
une:1959.11/30595
Abstract
Two studies were conducted to investigate the effect of Bacillus amyloliquefaciens CECT 5940 (BA) as a probiotic on growth performance, amino acid digestibility and bacteria population in broiler chickens under a subclinical necrotic enteritis (NE) challenge and/or fed diets with different levels of crude protein (CP). Both studies consisted of a 2 × 2 factorial arrangement of treatments with 480 Ross 308 mix-sexed broiler chickens. In study 1, treatments included 1) NE challenge (+/−), and 2) BA (1.0 × 106 CFU/g of feed) supplementation (+/−). In study 2, all birds were under NE challenge, and treatments were 1) CP level (Standard/Reduced [2% less than standard]) and 2) BA (1.0 × 106 CFU/g of feed) supplementation (+/−). After inducing NE infection, blood samples were taken on d 16 for uric acid evaluation, and cecal samples were collected for bacterial enumeration. In both studies, ileal digesta was collected on d 35 for nutrient digestibility evaluation. In study 1, the NE challenge reduced body weight gain (BWG), supressed feed conversion ratio (FCR) and serum uric acid levels (P < 0.001). Supplementation of BA increased BWG (P < 0.001) and reduced FCR (P = 0.043) across dietary treatments, regardless of challenge. Bacillus (P = 0.030) and Ruminococcus (P = 0.029) genomic DNA copy numbers and concentration of butyrate (P = 0.017) were higher in birds fed the diets supplemented with BA. In study 2, reduced protein (RCP) diets decreased BWG (P = 0.010) and uric acid levels in serum (P < 0.001). Supplementation of BA improved BWG (P = 0.001) and FCR (P = 0.005) and increased Ruminococcus numbers (P = 0.018) and butyrate concentration (P = 0.033) in the ceca, regardless of dietary CP level. Further, addition of BA reduced Clostridium perfringens numbers only in birds fed with RCP diets (P = 0.039). At d 35, BA supplemented diets showed higher apparent ileal digestibility of cystine (P = 0.013), valine (P = 0.020), and lysine (P = 0.014). In conclusion, this study suggests positive effects of BA supplementation in broiler diets via modulating gut microflora and improving nutrient uptake.
Link
Citation
Animal Nutrition, 7(1), p. 185-197
ISSN
2405-6383
2405-6545
Start page
185
End page
197
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International

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