Effects of auxins on organogenesis and somatic embryogenesis from juvenile explants of 'Eucalyptus erythronema', E. 'stricklandii', and two inter-specific hybrids

Glocke, Pauline; Collins, Graham; Sedgley, Margaret

Author(s)

Glocke, Pauline

Collins, Graham

Sedgley, Margaret

Publication Date

2006

Abstract

Organogenesis and somatic embryogenesis were investigated in apex and cotyledon explants of 'Eucalyptus erythronema, E. strick landii' and their inter-specific hybrids 'Eucalyptus erythronema' X E. 'stricklandii cv'. 'Urrbrae Gem' and 'Hybrid 2.5', following exposure to 1 mg 1-¹ naphthaleneacetic acid (NAA) plus 1 mg 1-¹ 2,4dichlorophenoxyacetic acid (2,4-0), or 3, 5 or 15 mg 1-¹ NAA alone. Somatic embryogenesis was not observed macroscopically; however, structures characteristic of globular somatic embryos or root primordia were observed using light microscopy of apex explants of 'Urrbrae Gem' seedlings after 14 d on Murashige and Skoog (1962) (MS) medium supplemented with 3 mg 1-¹ NAA. Root development was associated with explant vascular tissue and observed in all plant growth regulator (PGR) treatments, but was less on explants treated with 1 mg 1-¹ NAA plus 1 mg 1-¹ 2,4-0 than in all NAA-alone treatments. Shoot development was observed on apex explants after sub-culture on PGR-free medium, but was less after treatment with 1 mg 1-¹ NAA plus 1 mg 1-¹ 2,4-0 compared to all NAA-on1y treatments. Roots and sho ts developed simultaneously on apex explants after culture for 1 week on MS medium supplemented with 15 mg 1-¹NAA.

Citation

Journal of Horticultural Science and Biotechnology, 81(6), p. 1009-1014

ISSN

1462-0316

Link

https://hdl.handle.net/1959.11/4867

Language

en

Publisher

Headley Brothers Ltd

Title

Effects of auxins on organogenesis and somatic embryogenesis from juvenile explants of 'Eucalyptus erythronema', E. 'stricklandii', and two inter-specific hybrids

Type of document

Journal Article

Entity Type

Publication

Author(s)	Glocke, Pauline Collins, Graham Sedgley, Margaret
Publication Date	2006
Abstract	Organogenesis and somatic embryogenesis were investigated in apex and cotyledon explants of 'Eucalyptus erythronema, E. strick landii' and their inter-specific hybrids 'Eucalyptus erythronema' X E. 'stricklandii cv'. 'Urrbrae Gem' and 'Hybrid 2.5', following exposure to 1 mg 1-¹ naphthaleneacetic acid (NAA) plus 1 mg 1-¹ 2,4dichlorophenoxyacetic acid (2,4-0), or 3, 5 or 15 mg 1-¹ NAA alone. Somatic embryogenesis was not observed macroscopically; however, structures characteristic of globular somatic embryos or root primordia were observed using light microscopy of apex explants of 'Urrbrae Gem' seedlings after 14 d on Murashige and Skoog (1962) (MS) medium supplemented with 3 mg 1-¹ NAA. Root development was associated with explant vascular tissue and observed in all plant growth regulator (PGR) treatments, but was less on explants treated with 1 mg 1-¹ NAA plus 1 mg 1-¹ 2,4-0 than in all NAA-alone treatments. Shoot development was observed on apex explants after sub-culture on PGR-free medium, but was less after treatment with 1 mg 1-¹ NAA plus 1 mg 1-¹ 2,4-0 compared to all NAA-on1y treatments. Roots and sho ts developed simultaneously on apex explants after culture for 1 week on MS medium supplemented with 15 mg 1-¹NAA.
Citation	Journal of Horticultural Science and Biotechnology, 81(6), p. 1009-1014
ISSN	1462-0316
Link	https://hdl.handle.net/1959.11/4867
Language	en
Publisher	Headley Brothers Ltd
Title	Effects of auxins on organogenesis and somatic embryogenesis from juvenile explants of 'Eucalyptus erythronema', E. 'stricklandii', and two inter-specific hybrids
Type of document	Journal Article
Entity Type	Publication

Effects of auxins on organogenesis and somatic embryogenesis from juvenile explants of 'Eucalyptus erythronema', E. 'stricklandii', and two inter-specific hybrids

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