Development of modified apple polysaccharide capped silver nanoparticles loaded with mesalamine for effective treatment of ulcerative colitis

2020-12

Kaur, Gurmandeep

Singh, Sachin Kumar

Kumar, Rajesh

Kumar, Bimlesh

Kumari, Yogita

Gulati, Monica

Pandey, Narendra Kumar

Gowthamarajan, K

Ghosh, Dipanjoy

Clarisse, A

Wadhwa, Sheetu

Mehta, Meenu

Satija, Saurabh

Dua, Kamal

Dureja, Harish

Gupta, Saurabh

Singh, Pankaj Kumar

Kapoor, Bhupinder

Chitranshi, Nitin

Kumar, Ankit

Porwal, Omji

Journal Article

en

Publication

Elsevier BV

The Netherlands

10.1016/j.jddst.2020.101980

une:1959.11/71703

The objective of study was to develop modified apple polysaccharide (MAP) based silver nanoparticles (AgNPs) loaded with mesalamine (MES) for effective treatment of ulcerative colitis in acetic acid induced rat model. AgNPs were prepared by reducing silver nitrate using MAP solution. The size and zeta potential of AgNPs was 89 ± 3 nm and −16.3 ± 1.54 mV and AgNPs loaded with MES (AgNPs-MES) was 101 ± 9 nm and −14.27 ± 2.16 mV. The dissolution study revealed about 54% drug release after 5 h indicating release of drug at the colonic site. The in vivo study was carried out on acetic acid induced ulcerative colitis rats and efficacy of treatment was assessed through evaluation of disease activity index and level of antioxidants as well as tumor necrosis factor-α after 7th and 14th day of induction of colitis. Histopathological evaluation of colonic tissue was also carried out. The results revealed that AgNPs-MES (high dose) provided better therapeutic efficacy for the treatment of UC as compared to its low dose, MES alone, MES-MAP, AgNPs alone and MAP alone. It was concluded that MAP based AgNPs loaded with MES were successfully formulated and found to be effective in treating ulcerative colitis.

link

Journal of Drug Delivery Science and Technology, v.60

2588-8943

1773-2247