Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples

Author(s)
Renz, Katrin
Cheetham, Brian Frederick
Islam, Aminul
Walkden-Brown, Steve William
Publication Date
2006
Abstract
Methods for taqman real-time PCR assays to detect the three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes ofMDV on a sample. Absolute quantification of MDV2 in dust samples from poultry farms across Australia in a preliminary study, revealed the presence of MDV2 in 13 of 30 samples tested.
Citation
Proceedings of the Australian Poultry Science Symposium, v.18, p. 153-156
ISSN
1034-3466
1034-6260
Link
https://hdl.handle.net/1959.11/4348
Language
en
Publisher
University of Sydney, Poultry Research Foundation
Title
Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples
Type of document
Conference Publication
Entity Type
Publication

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