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Absolute quantification of Marek's disease virus serotype 2 (MDV2) using real-time polymerase chain reaction and its application to field dust samples |
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Cheetham, Brian Frederick |
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Editor(s): Dr Peter Selle |
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University of Sydney, Poultry Research Foundation |
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| Abstract |
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Methods for taqman real-time PCR assays to detect the three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes ofMDV on a sample. Absolute quantification of MDV2 in dust samples from poultry farms across Australia in a preliminary study, revealed the presence of MDV2 in 13 of 30 samples tested. |
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Proceedings of the Australian Poultry Science Symposium, v.18, p. 153-156 |
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